1.1.1 Organ
Aerial parts--early rosette

1.1.2 Amount
Untargeted metabolites were extracted from 100mg of fresh tissue

1.1.3 Growth condition
Arabidopsis thaliana ecotype Col-0, grown on 1/2MS petri plates under conditions as follows: 160µmol. m-2.sec-1 of light, 16-h light/8h dark, at 21°/19°C, with 65% relative air humidity).

1.1.4 Experimental condition
Transgenic plants grown alongside WT Col-0 expressing fungal-derived hydrolases targeting cell wall polysaccharides

1.1.5 Sampling and sampling date
Total aboveground portion of plants (early rosette stage) harvested at 14 days after planting.

1.1.6 Metabolism quenching method
Rosettes of each sample were harvested. All the plant materials were frozen immediately in liquid nitrogen to quench the enzymatic activity.

1.2 Chemical analysis metadata
1.2.1 Sample processing and extraction
samples were extracted from 100mg of fresh tissue with 50% methanol (v/v) at 60 C for 30 min. After centrifugation (10 min, 14 000 g), the supernatant was transferred to an HPLC vial. Metabolite profiles were obtained by liquid chromatography coupled with jet-stream electrospray ionization-quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS).

1.2.2 LC-MS conditions
Separation was performed on an Eclipse Plus C18 column (100 × 3 mm, 1.8 μm) under the following conditions: solvent A=water; solvent B=acetonitrile; flow rate =0.6 ml min–1; injection volume = 5 μl.

1.2.3 Data processing
Data were processed using Agilent profinder software to generate peak lists consisting of variables described by mass-to-charge ratio and retention time. Multivariate analysis was carried out in Agilent Mass Profiler Professional software.

References